| Autor: |
Vincent Croquette, H. Berthoumieux, T. Barilero, T. Le Saux, Annie Lemarchand, E. Cavatore, K. Zrelli, Charlie Gosse, Ludovic Jullien |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
2011 16th International Solid-State Sensors, Actuators and Microsystems Conference. |
| Popis: |
Temperature oscillations allow one to modulate the concentration of reagents and products with given amplitude and phase shift, both parameters depending on the dynamics of the chemical system under study. We here rely on thermal excitation in a microdevice, associated with fluorescence video microscopy as well as image filtering protocols, to selectively enhance the signal issued from a labeled probe that hybridizes with a targeted oligonucleotide. Furthermore, by simply tuning the stimulation parameters, focus can be put either on the perfect match sequence or on some other one that bears a point mutation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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