Genoprotection and Cytotoxicity of Cassia surattensis Seed Extract on Vero Cell Evaluated by Comet and Cytotoxicity Assays
| Autor: | Yeng Chen, Sreenivasan Sasidharan, Subramanion L. Jothy, Jagat R. Kanwar, Nowroji Kavitha, U. Seeta Uthaya Kumar |
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| Rok vydání: | 2016 |
| Předmět: |
Antioxidant
Cassia surattensis Traditional medicine medicine.medical_treatment In vitro cytotoxicity 0211 other engineering and technologies food and beverages 02 engineering and technology Biology biology.organism_classification Comet assay 03 medical and health sciences 0302 clinical medicine Cassia 030220 oncology & carcinogenesis 021105 building & construction Botany medicine Vero cell General Agricultural and Biological Sciences Cytotoxicity Medicinal plants General Environmental Science |
| Zdroj: | Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 88:313-320 |
| ISSN: | 2250-1746 0369-8211 |
| DOI: | 10.1007/s40011-016-0761-8 |
| Popis: | Cassia (C.) surattensis Burm. f. (Leguminosae) is a flowering plant that has been traditionally used in many countries for food and medicinal purposes. The present study was conducted to determine in vitro cytotoxicity and anti-genotoxic activity of the seed extract of C. surattensis. The cytotoxic and anti-genotoxic activities were evaluated against Vero cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and comet assays. In vitro cytotoxicity evaluation demonstrated that seed extract of C. surattensis was non-cytotoxic against the Vero cells. The 50 % cytotoxic concentration (CC50) value from MTT cytotoxicity assay was 332.87 µg/ml. The seed extract of C. surattensis exhibited strong inhibitory effects against H2O2-mediated DNA damage in Vero cells as observed in Comet assay. The presence of antioxidant activity in the seed extract was associated with the antigenotoxic property of C. surattensis. In summary, the present study demonstrated an efficient antigenotoxic property of C. surattensis, which is significant for future investigation for human applications. |
| Databáze: | OpenAIRE |
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