Organelle-Specific Isozymes of Citrate Synthase in the Endosperm of Developing Ricinus Seedlings

Autor:	Takeshi Kagawa, Elma González
Rok vydání:	1981
Předmět:	chemistry.chemical_classification biology Physiology Endoplasmic reticulum Ricinus Plant Science biology.organism_classification Molecular biology Isozyme Endosperm Enzyme chemistry Biochemistry Organelle Genetics biology.protein Citrate synthase
Zdroj:	Plant Physiology. 68:845-850
ISSN:	1532-2548 0032-0889
DOI:	10.1104/pp.68.4.845
Popis:	Chromatographic analysis of organelle-associated citrate synthase activity revealed distinct mitochondrial and glyoxysomal forms of the enzyme. The chromatographic elution patterns on hydroxylapatite, carboxymethylcellulose and DEAE-cellulose of citrate synthase from the endosperm of 4.5-day-old castor bean seedlings revealed significant differences for mitochondrial and glyoxysomal activities of the enzyme. The endoplasmic reticulum-associated citrate synthase activity eluted from DEAE-cellulose in a pattern that was identical to that of the glyoxysomal activity. The same kinds of organelle specific isozyme elution patterns were observed with young, developing seedlings. Gibberellic acid-treatment of young seedlings increased total recoverable citrate synthase activity from endosperm tissue but did not modify the organelle specific isozyme relationships.
Databáze:	OpenAIRE
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