Abstract 823: Identification of FISH biomarkers to detect chromosome abnormalities associated with prostate adenocarcinoma in tumor and field effect environment
| Autor: | Ying Zhang, Beth Blondin, Katerina Pestova, Thomas Perez, Larry E. Morrison, Diana Escarzaga, John S. Coon |
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| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Cancer Research. 70:823-823 |
| ISSN: | 1538-7445 0008-5472 |
| DOI: | 10.1158/1538-7445.am10-823 |
| Popis: | Introduction. The focal nature of prostate cancer (CA) contributes to needle biopsy sampling error with false negative rates of 15-30%. Here we used fluorescence in situ hybridization (FISH) analysis to assess molecular abnormalities in prostate specimens as an aid to CA diagnosis. We also evaluated the feasibility of combined immunofluorescence and FISH to facilitate assessment of chromosomal abnormalities. Experimental Procedure. 33 radical prostatectomy (RP) specimens from patients with adenocarcinoma of the prostate were evaluated by FISH with MYC, LPL, PTEN and centromere 8 probes. For each specimen, a tissue section was scribed by a pathologist to mark the tumor region(s). For 17 of the RP cases, a second section was available with only histologically benign tissue. FISH signals were enumerated in 50-100 cells per section. 26 hyperplasia (BPH) specimens, served as controls. Immunofluorescence (IF) with AMACR antibody was also used in the same assay with FISH. Results. Chromosomal copy number abnormalities were observed in most RP specimens, both within tumor regions and extending beyond histologically evident tumor, while few abnormalities were observed in the BPH specimens, using a cut-off value for FISH positivity based on the mean +/- 2 SD of cells with less than or greater than 2 signals in BPH specimens. A combination of MYC gain, LPL loss, PTEN loss or chromosome 8 aneusomy within the scribed tumor regions identified adenocarcinoma in 82% (27/33) of RP specimens, with a specificity of 81% relative to BPH (χ2 p Conclusions. This study identified FISH probes for detection of chromosomal abnormalities associated with prostate CA that could potentially serve as a diagnostic aid in biopsy specimens. This study also indicates that chromosome abnormalities are present in RP specimens within regions of normal histology, indicating a substantial tumor field effect. Therefore, a molecular test based on FISH to measure abnormal MYC, LPL, PTEN and centromere 8 copy numbers may allow detection of cancer otherwise missed by histopathological examination and thus improve diagnosis of prostate cancer by reducing sampling error of needle biopsies. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 823. |
| Databáze: | OpenAIRE |
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