Ethyl acetate extract of the Musa nana flower inhibits osteoclastogenesis and suppresses NF-κB and MAPK pathways

Autor:	Xue-Ming Zhou, Min-Min Tang, Jia-Ying Wu, Cai Jin, Guang-Ying Chen, Ying-Jie Chen, Aftab Amin, Zhi-Ling Yu
Rok vydání:	2021
Předmět:	MAPK/ERK pathway Effector fungi food and beverages NF-κB General Medicine Pharmacology Quercitrin Bone resorption chemistry.chemical_compound medicine.anatomical_structure chemistry Polyphenol Osteoclast medicine Quercetin Food Science
Zdroj:	Food & Function. 12:11586-11598
ISSN:	2042-650X 2042-6496
DOI:	10.1039/d1fo02204k
Popis:	Banana flowers are consumed as a vegetable and traditionally used for managing several health problems including joint pain, a symptom of bone loss. Osteoclasts are key effector cells responsible for bone loss. Some flavonoids in banana flowers, such as quercetin and quercitrin, have been shown to be able to inhibit osteoclastogenesis. Whether banana flowers can inhibit osteoclast formation is unknown. In this study, we prepared the ethyl acetate fraction (FFE-EA) of an ethanolic extract of fresh flowers of Musa nana. Using UPLC-MS/MS analyses, 76 polyphenols were identified in FFE-EA. In RANKL-stimulated RAW264.7 macrophages, FFE-EA inhibited osteoclastogenesis and osteoclastic bone resorption. Mechanistic studies revealed that FFE-EA suppressed NF-κB and MAPK pathways, and lowered mRNA levels of osteoclast formation/function-related genes. These findings suggest that flowers of M. nana could be a source for formulating functional food that benefits bone health.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::29a35a8378658a6433e3368a7f22e1b4 https://doi.org/10.1039/d1fo02204k Zobrazit plný text záznamu