Detection of mutations in ampC promoter/attenuator gene in Escherichia coli from dairy cows in Rio de Janeiro and Mato Grosso, Brazil
| Autor: | Stefaninni Santiago Gabrielli, da Silva Coelho Irene, Oliveira de Farias Beatriz, Abreu de Alencar Tatiani, Borges Moreira Amy, França Bronzato Greiciane, Moreira Soares de Souza Miliane, Gomes de Castro Bruno, Neto Ferreira Helena, de Mattos de Oliveira Coelho Shana |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine 030106 microbiology Attenuator (genetics) Plant Science Biology Gene mutation medicine.disease_cause 01 natural sciences Microbiology DNA sequencing 03 medical and health sciences 010608 biotechnology polycyclic compounds medicine Cefoxitin Gene Escherichia coli biochemical phenomena metabolism and nutrition bacterial infections and mycoses medicine.disease Phenotype Mastitis Infectious Diseases bacteria medicine.drug |
| Zdroj: | African Journal of Microbiology Research. 13:388-391 |
| ISSN: | 1996-0808 |
| Popis: | Escherichia coli present the ampC naturally, and the observation of phenotypical resistance to cefoxitin is related to this gene deregulation. Mutations in the regulatory region in ampC cause exaggerated expression. The most frequent alterations in the E. coli AmpC promoter/attenuator leading to this overexpression is described at the positions: -88, -82, -42, -18, -1 and +58. Mastitis studies were carried in Rio de Janeiro and Mato Grosso, Brazil. Two cefoxitin and amoxicillin-clavulanic acid-resistant E. coli from farms animals were unusually detected once these characteristics are not observed together in this species. The objective of this work was to determine if these isolates had a chromosomal gene mutation, determining AmpC hyperproduction. After DNA sequencing, mutations were observed at -88, -82, -73, -18, -1 and +58 positions, confirming the initially suspected AmpC hyperexpression. In Brazil, this is the first work to report E. coli hyperproducing this enzyme. Key words: Ampc attenuator, AmpC hyperproduction, ampC promoter, bovine feces, mastitic milk. |
| Databáze: | OpenAIRE |
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