Disruption and functional analysis of six ORFs on chromosome XII ofSaccharomyces cerevisiae:YLR124w,YLR125w,YLR126c,YLR127c,YLR128w andYLR129w

Autor:	Wei Cui, Martin Willer, John R. Tyson, Peter J. Reid, Colin J. Stirling, Barrie Wilkinson, Matthieu Regnacq
Rok vydání:	2000
Předmět:	Genetics Saccharomyces cerevisiae Chromosome Bioengineering Context (language use) Biology biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Molecular biology Open reading frame Plasmid Ploidy ORFS Gene Biotechnology
Zdroj:	Yeast. 16:1429-1435
ISSN:	1097-0061 0749-503X
DOI:	10.1002/1097-0061(200011)16:15<1429::aid-yea629>3.0.co;2-s
Popis:	In the context of the EUROFAN programme, we report the deletion and functional analysis of six open reading frames (ORFs) on the right arm of chromosome XII of Saccharomyces cerevisiae. Using a PCR-based gene replacement strategy, we have systematically deleted individual ORFs and subjected the heterozygous diploids and haploid knockout strains to basic genetic and phenotypic characterization. Two ORFs, YLR127c and YLR129w, are essential for viability, whereas no growth phenotype could be detected following deletion of YLR124w, YLR125w, YLR126c or YLR128w. For each of the individual ORFs, a kanMX4 replacement cassette and the corresponding cognate wild-type gene were cloned into appropriate plasmids. Copyright © 2000 John Wiley & Sons, Ltd.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::298e6568665fbec74587c686a7b86eca https://doi.org/10.1002/1097-0061(200011)16:15<1429::aid-yea629>3.0.co Zobrazit plný text záznamu Plný text