Hydrolytic and oxidative enzyme production through cultivation ofPleurotus ostreatuson pulp and paper industry wastes
| Autor: | Andrej Gregori, Kristina Sepčić, Matej Skočaj, Maja Grundner, Mija Sežun |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology Chemistry Triacylglycerol lipase Cellulase biology.organism_classification 01 natural sciences Enzyme assay Biomaterials 03 medical and health sciences 030104 developmental biology 010608 biotechnology Oxidative enzyme biology.protein Xylanase Fermentation Pleurotus ostreatus Food science Lipase |
| Zdroj: | Holzforschung. 72:813-817 |
| ISSN: | 1437-434X 0018-3830 |
| Popis: | The growth of oyster mushroom (Pleurotus ostreatus) on pulp and paper industry wastes was studied. Specifically, the question was investigated whether solid-state fermentation ofP. ostreatuson paper-mill deinking sludge and primary sludge substrates is appropriate for production of enzymes, relevant to the pulp and paper industry. Following fermentation, extracellular protein was extracted and the specific activities of four enzymes were determined, namely, the cellulase, xylanase, lipase and peroxidase. Furthermore, the effects of the pH of the extraction buffer on these enzyme activities were determined, along with the effects of the incubation time. The data show thatP. ostreatuscan grow on solid wastes from the pulp and paper industry, which could help to minimize the waste volume and to decrease the ecological impact. Furthermore, the solid wastes in focus are good substrates for the production of commercially interesting enzymes. |
| Databáze: | OpenAIRE |
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