Differential expression of cancer pathway-related genes in low-versus high-dose-rate-irradiated AKR/J mice

Autor:	Hee Sun Kim, Seung Jin Choi, Suk Chul Shin, Jin Jong Bong, Yu Mi Kang, Moo Hyun Choi
Rok vydání:	2012
Předmět:	Nuclear and High Energy Physics Radiation TUNEL assay Microarray Cumulative dose Chemistry Cancer Condensed Matter Physics medicine.disease Molecular biology Ionizing radiation Real-time polymerase chain reaction Apoptosis medicine Anisocytosis General Materials Science
Zdroj:	Radiation Effects and Defects in Solids. 167:863-870
ISSN:	1029-4953 1042-0150
DOI:	10.1080/10420150.2012.701218
Popis:	To understand the biological effects of ionizing radiation on lymphomagenesis, we reared AKR/J mice for 130 days with exposure to either high-dose-rate (HDR, 0.8 Gy/min, a single dose of 4.5 Gy) or low-dose-rate (LDR, 0.7 mGy/h, a cumulative dose of 2.1 Gy) irradiation. After 130 days, we compared the mean thymus weight, analyzed the histological changes, and measured apoptotic cell numbers using the terminal deoxynucleotidyl transferase-mediated dUTP-end labeling (TUNEL) assay. We also used microarrays and quantitative polymerase chain reaction analysis (qPCR) to analyze the expression profiles of cancer pathway-related genes in the thymuses of the mice. The mean thymus weight of the LDR-irradiated mice decreased relative to Sham- and HDR-irradiated mice. Histopathological examination revealed that the neoplastic cells in the thymuses of the Sham- and HDR-irradiated mice were pleomorphic, with marked anisocytosis and anisokaryosis, whereas the cells and their nuclei were relatively small and uniform in s...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::296bf870f5fa28155872adf3fd4001cf https://doi.org/10.1080/10420150.2012.701218 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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