Intrasplenic vaccination against experimental melanoma

Autor:	Nichola Kouttab, David Shrayer, S Paul, Harold J. Wanebo, C Park, Hearing, Hendrix Bogaars, R McInnis, Abby Maizel
Rok vydání:	1996
Předmět:	Cancer Research biology Lymphokine chemical and pharmacologic phenomena Immune system Oncology Antigen Apoptosis Immunology biology.protein Cancer research Splenocyte Cytotoxic T cell IL-2 receptor Antibody
Zdroj:	International Journal of Oncology.
ISSN:	1791-2423 1019-6439
DOI:	10.3892/ijo.9.1.123
Popis:	The immunodominant component of a formalinized extracellular antigen (fECA) vaccine prepared from B16 F10 melanoma cells is the melanoma-associated antigen B700. We now demonstrate that a single prophylactic intrasplenic inoculation of B700 antigen (1-10 mu g) stimulates the production of antibodies which have antiproliferative effects on B16 F10 melanoma cells in vitro. In addition, potential cytotoxic effects of splenocytes from B700 antigen inoculated mice were evaluated for two cellular immune effector functions, natural killer (NK) cell activity and lymphokine activated killer (LAK) cell activity; both activities were increased following B700 antigen inoculation. Intrasplenic injection of B700 antigen elicited an increase in the expression of the CD25 surface antigen (IL-2 R alpha) by T lymphocytes and up-regulated the expression of IL-2 R alpha mRNA. Thus both humoral and cellular cytotoxic immune responses might play roles in the decreased growth of primary tumors in B700 antigen inoculated mice and in the higher survival rate in this group of animals.
Databáze:	OpenAIRE
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