Effect of Organic Substrate on the Microbial Community Structure in Pilot-Scale Sulfate-Reducing Biochemical Reactors Treating Mine Drainage

Autor:	Sage R. Hiibel, Luciana P. Pereyra, David J. Reisman, Amy Pruden, Kenneth F. Reardon, Maria V. Riquelme Breazeal
Rok vydání:	2011
Předmět:	Waste management Sulfur cycle Biology Pulp and paper industry complex mixtures Pollution Substrate (marine biology) chemistry.chemical_compound Bioremediation Corn stover Microbial population biology chemistry Microbial ecology Environmental Chemistry Sulfate Drainage Waste Management and Disposal
Zdroj:	Environmental Engineering Science. 28:563-572
ISSN:	1557-9018 1092-8758
Popis:	Passive biological systems such as sulfate-reducing biochemical reactors have shown promise for treatment of mine drainage because of their low cost, minimal maintenance, and constructability in remote locations. However, few criteria exist for their design and operation. In particular, the impact of the choice of carbon substrate is poorly understood. This study represents the first to directly compare the effect of simple and complex organic substrate on microbial communities present in pilot-scale biochemical reactors treating mine drainage. Three organic substrates were evaluated: ethanol (ETOH), hay and pine wood chips (HYWD), and corn stover and pine wood chips (CSWD). Microbial community compositions were characterized by cloning and sequencing of 16S rRNA and apsA genes corresponding to the sulfur cycle. Quantitative polymerase chain reaction was applied to quantify Desulfovibrio-Desulfomicrobium spp. and methanogens. Results revealed differences in microbial compositions and relative qua...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::29631128ba85b9a08bbe32f849541df3 https://doi.org/10.1089/ees.2010.0237 Zobrazit plný text záznamu