Activated naïve B cells promote development of malignant pleural effusion by differential regulation of TH1 and TH17 response
| Autor: | Xiu-Zhi Wu, Zheng Wang, Xue-Bin Pei, Kan Zhai, Xin-Yu Shi, Feng-Shuang Yi, Huan-Zhong Shi, Wen Wang, Zhen Wang, Li-Li Xu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine CD86 Adoptive cell transfer biology Physiology Chemistry Cell Naive B cell CD44 Spleen Cell Biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Immune system Physiology (medical) Cancer research medicine biology.protein CD80 030215 immunology |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 315:L443-L455 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.00120.2018 |
| Popis: | Inflammatory signaling networks between tumor cells and immune cells contribute to the development of malignant pleural effusion (MPE). B cells have been found in MPE; however, little is known about their roles there. In the present study, by using mouse MPE models, we noted that although the total B cells in MPE were decreased as compared with the corresponding blood and spleen, the percentage of activated naïve B cells expressing higher levels of CD80, CD86, myosin heavy chain-II, CD44, CD69, and programmed cell death-ligand 1 (PD-L1) molecules were increased in wild-type mouse MPE. Compared with wild-type mice, decreased T helper (TH)1 cells and increased TH17 cells were present in B cell-deficient mouse MPE, which paralleled to the reduced MPE volume and longer survival time. Adoptive transfer of activated naïve B cells into B cell-deficient mice was able to increase TH1 cells and decrease TH17 cells in MPE and shorten the survival of mice bearing MPE. Furthermore, we demonstrated that activated naïve B cells inhibited TH17-cell expansion via the PD-1/PD-L1 pathway and promoted naïve CD4+ T-cell differentiation into TH1/TH17 cells through secreting IL-27/IL-6 independent of the PD-1/PD-L1 pathway. Collectively, our data uncovered a mechanism by which naïve B cells promote MPE formation by regulating TH1/TH17 cell responses, making these B cells an attractive target for therapeutic intervention in the fight against cancer. |
| Databáze: | OpenAIRE |
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