Chromosome banding and FISH with rDNA probe in the diploid and tetraploid loach Misgurnus anguillicaudatus

Autor:	Ping-Ping Tian, Zhuo Yu, Yi Tian, Syuiti Abe, Katsutoshi Arai, Ya-Juan Li, Ming-Zhao Zhang
Rok vydání:	2010
Předmět:	Genetics Cobitidae medicine.diagnostic_test biology Weatherfish Misgurnus Karyotype biology.organism_classification Molecular biology chemistry.chemical_compound chemistry medicine Chromomycin A3 Ploidy Ribosomal DNA Ecology Evolution Behavior and Systematics Fluorescence in situ hybridization
Zdroj:	Ichthyological Research. 57:358-366
ISSN:	1616-3915 1341-8998
DOI:	10.1007/s10228-010-0168-0
Popis:	The chromosomes of the diploid and tetraploid loach Misgurnus anguillicaudatus were analyzed by staining with Ag, chromomycin A3 (CMA3)/distamycin A (DA), and DA/4′,6-diamidino-2-phenylindole (DAPI), and using fluorescence in situ hybridization (FISH) with 5.8S + 28S rDNA as a probe. Nucleolus organizer regions (NORs) were mapped to the telomeric region of the short arms of the largest (first) metacentric chromosome pair in the diploid loach with 2n = 50 and the homologous quartet in the tetraploid loach with 4n = 100. The NORs were positive at the same region of the first metacentric chromosome for Ag and CMA3/DA stainings, but negative for DA/DAPI staining. Four signals at the homologs within the same quartet suggest the duplication of the entire genome from diploid to tetraploid status. However, a size difference was detected between the rDNA signals by FISH and CMA3 banding.
Databáze:	OpenAIRE
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