Evidence That Luteinizing Hormone-Releasing Hormone Statin from Ovine Rete Testis Fluid Is Immunologically Related to αC Inhibin1
| Autor: | J. C. Poirier, B. Calas, Alain Locatelli, Jean-Louis Dacheux, Blanc Mr, C. Cariou-Guennoc |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
endocrine system
medicine.medical_specialty Statin medicine.drug_class Protein subunit Biological activity Cell Biology General Medicine Biology Testicle medicine.anatomical_structure Endocrinology Reproductive Medicine Affinity chromatography Internal medicine medicine biology.protein Antibody Luteinizing hormone hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Biology of Reproduction. 62:1551-1563 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod62.6.1551 |
| Popis: | LHRH Statin is a putative gonadal protein that increases the interval between two consecutive LHRH pulses. The present work was aimed at analyzing the immunological homology between LHRH Statin and the N-terminal region of the αC subunit of inhibin. Thus, rete testis fluid (RTF) proteins were purified by immunoaffinity chromatography using antibodies against residues 1–7 plus 7–30 (experiment 1, A-fractions) and 14–28 of the αC inhibin subunit (experiment 2, B-fractions), and the LHRH Statin activity of the fractions was examined by intracerebroventricular administration in castrated rams followed by RIA of plasma LH levels in 15-min blood samples. Fractions that bound to the immunoaffinity column with low affinity were eluted with 0.5 M NaCl, pH 7.4 (-F2); then highly bound fractions were eluted sequentially in acidic (pH 2.5, -F3) followed by basic conditions (pH 11.5, -F4). In experiment 1, RTF (40 μg, n = 4) and highly bound fractions (A-F3, 30 ng, n = 8, 150 ng, n = 3; A-F4, 120 ng, n = 5) d... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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