Pre-column derivatization of aminoglycosides with 1-fluoro-2,4-dinitrobenzene

Autor:	C.J.L.R. Govers, C.W. Mijnsbergen, A. Hulshoff, Dirk M. Barends, J.S. Blauw
Rok vydání:	1985
Předmět:	Chromatography Organic Chemistry 1-Fluoro-2 4-dinitrobenzene Side reaction Phthalate Hydrochloric acid General Medicine Phosphate Biochemistry Analytical Chemistry chemistry.chemical_compound chemistry Reagent Pre column derivatization Derivatization
Zdroj:	Journal of Chromatography A. 322:321-331
ISSN:	0021-9673
DOI:	10.1016/s0021-9673(01)97691-5
Popis:	The reaction between to bramycin and 1-fluoro-2,4-dinitrobenzene was studied. This reaction shows a sharp pH optimum because, even under moderate alkaline conditions, derivatization of the aliphatic hydroxyl groups of tobramycin becomes an important side reaction. Phosphate and phthalate buffers also react with the derivatization reagent. Buffers comprising tertiary amines and hydrochloric acid are preferred.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::28f98b1499ddbe16834e98d2df957dfd https://doi.org/10.1016/s0021-9673(01)97691-5 Zobrazit plný text záznamu