Identification and purification of the specific antigen ofParacoccidioides brasiliensisresponsible for immunoelectrophoretic band E

Autor:	D. Bout, Frida Naquira, L.A. Yarzabal, J. Fruit, Suzanne Andrieu
Rok vydání:	1977
Předmět:	Paracoccidioides brasiliensis Antiserum biology Inoculation General Medicine biology.organism_classification Molecular biology Microbiology Immunodiffusion Infectious Diseases Antigen Alkaline phosphatase Immunoadsorption Mycelium
Zdroj:	Medical Mycology. 15:79-85
ISSN:	1460-2709 1369-3786
Popis:	A new purified antigen (E2) of Paracoccidioides brasiliensis mycelial growth phase was isolated by immunoadsorption from a crude metabolic soluble extract of the fungus. The antiserum prepared in a rabbit by inoculation of E2 antigen developed only one immunodiffusion line with the crude metabolic extract. Findings on immunological analysis showed that E2 antigen is the antigenic component of immunoelectrophoretic band E. The isolated antigens did not possess detectable alkaline phosphatase activity. It reacted in immunodiffusion tests with all the sera (14/14) from P. brasiliensis infected patients containing precipitating antibodies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::28eb9ac00e74e3d45d30bde79090f715 https://doi.org/10.1080/00362177785190131 Zobrazit plný text záznamu