Vitamin B6 cofactor conjugated rhodamine 6G derivative: Fluorescent turn-on sensing of Al(III) and Cr(III) with bioimaging application in live HeLa cells
| Autor: | Suban K. Sahoo, Priyankar Paira, Yachana Upadhyay, S. K. Ashok Kumar, Rajender Kumar, Heung-Jin Choi |
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| Rok vydání: | 2019 |
| Předmět: |
biology
010405 organic chemistry Metal ions in aqueous solution Conjugated system 010402 general chemistry biology.organism_classification 01 natural sciences Fluorescence Binding constant Cofactor 0104 chemical sciences Inorganic Chemistry Rhodamine 6G HeLa chemistry.chemical_compound chemistry Materials Chemistry biology.protein Physical and Theoretical Chemistry Pyridoxal Nuclear chemistry |
| Zdroj: | Inorganica Chimica Acta. 489:198-203 |
| ISSN: | 0020-1693 |
| DOI: | 10.1016/j.ica.2019.02.028 |
| Popis: | This manuscript introduced a new optically active chemosensor L containing rhodamine 6G as the fluorogenic unit and the vitamin B6 cofactor pyridoxal 5′-phosphate (PLP) as the metal ions binding unit. Sensor L showed a significant turn-on fluorescence at 550 nm selectively in the presence of Al3+ and Cr3+ ions due to the complexation prompted opening of the spirocyclic ring. Using sensor L, the concentration of Al3+ and Cr3+ ions can be detected down to 0.41 µM and 2.33 µM, respectively. Sensor L formed a metal complex in 1:1 binding stoichiometry in solution with the estimated binding constant of 11,310 M−1 and 8373 M−1 with Al3+ and Cr3+, respectively. The probable 3D structure and the electronic properties of L and its complexes were proposed by semi-empirical PM6 method. Further, the bioimaging experiments were performed to examine the potential of L to detect the intracellular Al3+ and Cr3+ ions present in the live HeLa cells. |
| Databáze: | OpenAIRE |
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