Isosorbide spacer containing gemini surfactants: surface and biochemical study
| Autor: | Ranjitsinh V. Devkar, Sarmita Jana, Sarita Gupta, Bhavesh Mistry, Sanjeev Kumar, Tejal Gajaria, Kushan Parikh |
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| Rok vydání: | 2015 |
| Předmět: |
Isosorbide
Chromatography Polymers and Plastics Chemistry Cationic polymerization Krafft temperature Surface tension chemistry.chemical_compound Colloid and Surface Chemistry Bromide Critical micelle concentration Agarose gel electrophoresis Materials Chemistry medicine Physical and Theoretical Chemistry Solubility medicine.drug Nuclear chemistry |
| Zdroj: | Colloid and Polymer Science. 293:1437-1446 |
| ISSN: | 1435-1536 0303-402X |
| Popis: | Three novel sugar (isosorbide (isb)) spacer-based cationic gemini surfactants (m-isb-m, m = 12, 14 or 16 denoted as 7–9, respectively) have been synthesized and characterized. Micellization and surface properties of 7–9 are obtained by surface tension and conductivity measurements. Critical micelle concentration (CMC) and surface parameter data demonstrate the superiority over conventional polymethylene spacer-based gemini surfactants. Presence of isb as a spacer causes lowering of Krafft temperature (and increase of solubility). Plasmid DNA binding with 7–9 has been reported using agarose gel electrophoresis at different N+/P− molar ratios. Gemini 7 showed better DNA binding over others (8 or 9). Comparative cytotoxicity analyses, using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for normal (L132) and cancerous (A549) human lung cells, have revealed that gemini 9 is having a potential to treat the A549 without affecting L132 at the same concentration. Geminis (7–9) with different N+/P molar ratios (0.2 μg DNA fixed) act as cell viable media for A549 and could be used as a potential vector for gene transfection. Gemini 7 shows similar potential even at higher fixed value of DNA (1.0 μg). However, 8 and 9 work oppositely. |
| Databáze: | OpenAIRE |
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