Prototype of micro flow cell for multi-analyte sandwich immunoassay

Autor:	K. Akahori, Shuichi Shoji, Hirotaka Sato, Y. Ashihara, N. Honda, M. Isomura
Rok vydání:	2002
Předmět:	business.product_category Chromatography Materials science Check valve medicine.diagnostic_test Pressure control Microfluidics Surface micromachining Flow control (fluid) Flow velocity Reagent Immunoassay medicine business
Zdroj:	Digest of Papers. Microprocesses and Nanotechnology 2001. 2001 International Microprocesses and Nanotechnology Conference (IEEE Cat. No.01EX468).
DOI:	10.1109/imnc.2001.984048
Popis:	A micro flow cell for sandwich immunoassay using Teflon (Asahi Glass Co.: CYTOP/sup TM/) check valves for sample and reagent inlets is designed and fabricated. The check valves are used not only to prevent reverse flow of injected solutions but also to realize sequential injection of the sample and reagent by simple pressure control. The valve structure is designed to control the width and thickness of the sample and reagent flows at relatively small flow velocity that is useful to reduce effective sample and reagent volumes. We examined a fluorescent sandwich immunoassay for human AFP (/spl alpha/-fetoprotein) as a preliminary experiment and faster immunoassay within 30 min compared to the conventional one was realized in the micro flow cell.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::28a4982c79b21925a7f6ef284f17a6f2 https://doi.org/10.1109/imnc.2001.984048 Zobrazit plný text záznamu