Effect of Chronic Exposure to Ozone and Nitric Acid on Cytochrome P-450 Monooxygenase System of Rat Lung and Liver
| Autor: | Ram K. Sindhu, William J. Mautz, Yutaka Kikkawa, Nai-San Wang, Ichiro Fujita |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Chronic exposure
medicine.medical_specialty Ozone Lung Cytochrome biology Health Toxicology and Mutagenesis Monooxygenase Toxicology chemistry.chemical_compound Endocrinology medicine.anatomical_structure chemistry Biochemistry Nitric acid Internal medicine Monoclonal medicine biology.protein Benzphetamine medicine.drug |
| Zdroj: | Inhalation Toxicology. 8:695-708 |
| ISSN: | 1091-7691 0895-8378 |
| DOI: | 10.3109/08958379609005448 |
| Popis: | Male F344/N rats were exposed to 0.15 ppm ozone (O3) and 50 μg/m3 nitric acid (HNO3) vapor alone and in combination for 4 h/day, 3 days/wk for a total of 40 wk to investigate the effects of these environmental pollutants on cytochrome P-450 monooxygenases in pulmonary and hepatic microsomes. Exposure to HNO3 vapor alone caused a significant increase of 7-ethoxyresorufin O-deethylase (EROD) activity in the hepatic microsomes (p < .01). Immunoinhibition assays with monoclonal anti-cytochrome P-450 (CYP) 1A1/1A2 (1-7-1) showed that the monoclonal antibody-inhibitableEROD activity in the hepatic microsomes was increased by 59% (p < .01) and 40% (p < .05), respectively, in the HNO3 vapor and O3 plus HNO3 vapor groups. Benzphetamine N-demethylase activity was not significantly affected by exposure to either O3 or HNO3 vapor in the hepatic microsomes. However, in the lung, O3 exposure caused a 72% increase in benzphetamine N-demethylase activity (p < .01), whereas this activity was decreased by 15% by HN... |
| Databáze: | OpenAIRE |
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