| Autor: | Maria Regina Moretto, Cilmery Suemi Kurokawa, Rafaelle Batistella Pires, José Roberto Fioretto, Rossano César Bonatto, Cristina Maria Teixeira Fortes, Mario Ferreira Carpi, João Pessoa Araújo Júnior, Susiane de Oliveira Klefens, Marcos Aurélio de Moraes |
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| Rok vydání: | 2013 |
| Předmět: |
Mechanical ventilation
medicine.medical_specialty medicine.diagnostic_test business.industry medicine.medical_treatment Immunology Hematology Oxygenation respiratory system Pulmonary compliance Lung injury Biochemistry Gastroenterology Lesion Bronchoalveolar lavage Internal medicine medicine Breathing Immunology and Allergy medicine.symptom business Molecular Biology Saline |
| Zdroj: | Cytokine. 63:279 |
| ISSN: | 1043-4666 |
| DOI: | 10.1016/j.cyto.2013.06.156 |
| Popis: | Recently, HMGB1, a macrophage cytokine and nonhistone chromatin-associated protein, has been associated with sustained inflammatory response and production of cytokines in lung injury. Therefore, the aim of this study was to measure HMGB1 and TNF-alpha levels in bronchoalveolar lavage from rabbits with severe acute lung injury (ALI) induced by tracheal infusion of warm saline. Methods: Thirty rabbits were instrumented and randomly assigned into two groups: (1) animals with no ALI (Control Group-CG; n = 15); and (2) animals with severe ALI (Severe Group-SG; n = 15). The groups were ventilated during 4 h with CMV. After ventilation, bronchoalveolar lavage fluid (BAL) was collected so that HMGB1, TNF-alpha levels and percentage of white cell were determined. Left lung was collected for histological analysis and confirmation of lesion. Results: Lung injury decreased pulmonary compliance (SG before: 1.86 ± 0.576 > SG after: 0.67 ± 0.24) and oxygenation (PaO 2 – SG before: 427.92 ± 89.90 > SG after: 68.18 ± 19.08 mmHg). Higher levels of HMGB1 were found in SG group as compared to CG group [SG: median-62 (p25–p75 = 20–79) > CG: 15 (13–25) ng/mL; p = 0.035] and TNF-alpha level was similar to HMGB1 level in SG and CG groups [SG: 1078 (381–1224) > CG: 45 (25–86) pg/mL; p ⩽ 0.001]. HMGB1 and TNF-alpha showed inverse correlation with oxygenation and positive correlation between them. Neutrophil percentage and histological score were higher in SG than CG ( p = 0.001 and p = 0.0032, respectively). Conclusions: HMGB1 and TNF-alpha are found in alveolar space and correlation is found between them and with oxygenation in this experimental model. Fapesp grants: 2011/15144-2. |
| Databáze: | OpenAIRE |
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