| Popis: |
The decay in fluorescence anisotropy following an excitation pulse can be monitored using time-correlated single photon counting, and used to measure molecular rotation and homo-FRET in biomedical research. In this chapter we review the basis of polarized fluorescence emission, and how this emission can be detected and quantified to yield a time-resolved anisotropy curve. We then review chemical, instrumental and biological factors that can influence the shape and magnitude of anisotropy curves. Understanding the influence of these factors can then be used to aid in the interpretation of biomedical experiments using time-resolved anisotropy. |
