Ag/Au Bimetallic Nanoparticles Inhibit Tumor Growth and Prevent Metastasis in a Mouse Model
| Autor: | Anna Lyberopoulou, Nadiia Vityuk, Maria Gazouli, Efstathios P. Efstathopoulos, Iuliia Mukha, Charalampos Tsoukalas, George Theodoropoulos, Andreas C. Lazaris, Hector Katifelis, Penelope Bouziotis |
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| Rok vydání: | 2020 |
| Předmět: |
Biodistribution
Biophysics Pharmaceutical Science Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Metastasis Biomaterials Western blot In vivo Drug Discovery medicine Tumor growth Lung medicine.diagnostic_test Chemistry Organic Chemistry Cancer General Medicine 021001 nanoscience & nanotechnology Ligand (biochemistry) medicine.disease 0104 chemical sciences medicine.anatomical_structure Cancer research 0210 nano-technology |
| Zdroj: | International Journal of Nanomedicine. 15:6019-6032 |
| ISSN: | 1178-2013 |
| DOI: | 10.2147/ijn.s251760 |
| Popis: | Purpose To evaluate the antitumor efficacy of Ag3Au1Trp1:2NPs in a SCID mouse cancer model, with respect to their effect on tumor growth, on tumor's metastatic potential and the underlying molecular mechanism. Subjects and methods Ag3Au1Trp1:2NPs were radiolabeled with Gallium-68 and the biodistribution was studied in Swiss mice without tumors and in SCID mice bearing tumors. SCID mice received intratumoral Ag3Au1Trp1:2NPs and tumor size was measured using calipers. Lung and liver tissues were extracted and studied microscopically for the detection of any metastatic sites. Changes in the Caspase-3 and TNF-related apoptosis-inducing ligand (TRAIL) were also investigated using real-time PCR and Western blot techniques, respectively. Results In the 4T1 tumor-bearing SCID mice, Ag3Au1Trp1:2NPs showed quick passive accumulation at tumor sites at 30 mins post-injection. Mice that received the highest dose of NPs (5.6mg/mL) demonstrated a 1.9-fold lower tumor volume compared to that of the control group at 11 days post-injection, while mice that did not receive NPs showed metastatic sites in liver and lung. Extracted tumor tissue of treated mice revealed increased Casp-3 mRNA levels as well as elevated TRAIL protein levels. Conclusion Based on our results, Ag3Au1Trp1:2NPs express anti-tumor and anti-metastatic effects in vivo. Ag3Au1Trp1:2NPs also reach tumor site via the enhancement and retention effect which results in the apoptotic death of cancerous cells selectively via the extrinsic TRAIL-dependent pathway. |
| Databáze: | OpenAIRE |
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