Creation of artifactual internal membranes during fixation of Methanobacterium thermoautotrophicum
| Autor: | Peter Schönheit, Henry C. Aldrich, Dieter B. Beimborn |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
biology
Vesicle education Immunology General Medicine biology.organism_classification Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Membrane Mesosome chemistry Osmium tetroxide Biochemistry Genetics Nucleoid Glutaraldehyde Molecular Biology Bacteria Fixation (histology) |
| Zdroj: | Canadian Journal of Microbiology. 33:844-849 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/m87-148 |
| Popis: | Methanogenic bacteria chemically fixed for electron microscopy contain internal membranes in the form of mesosomes and nucleoid vesicles, and the function of these membranes in methanogenesis and ATP synthesis has been speculated upon. Cells of Methanobacterium thermoautotrophicum fixed in phosphate-buffered 2.5% glutaraldehyde, 0.1% osmium tetroxide, or 0.5% glutaraldehyde – 2.5% formaldehyde always contain internal membranes. Cells freeze-fractured, freeze-substituted, or fixed in cacodylate-buffered 1% osmium tetroxide, 2.5% glutaraldehyde, or simultaneous glutaraldehyde–osmium lack internal membranes. We conclude that internal membranes in this species are artifacts arising during suboptimal fixation conditions. |
| Databáze: | OpenAIRE |
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