Monitoring of mammalian cell growth and virus production process using on-line oxygen uptake rate measurement

Autor:	Weichang Zhou, Wei Shou Hu, David M. Gryte, Cheryl M. Kussow
Rok vydání:	1995
Předmět:	Lysis Cell growth chemistry.chemical_element Microcarrier Bioengineering Biology Applied Microbiology and Biotechnology Biochemistry Oxygen Virus Microbiology Titer chemistry Cell culture Bioreactor Biophysics Biotechnology
Zdroj:	Enzyme and Microbial Technology. 17:779-783
ISSN:	0141-0229
DOI:	10.1016/0141-0229(94)00035-p
Popis:	Porcine epithelial cells were cultivated on microspheres with an average diameter of 35 μm in a stirred tank bioreactor for virus production. The oxygen uptake rate (OUR) was determined on-line by a dynamic method. This on-line measurement was used to estimate glucose consumption rate for perfusion rate adjustment. Using this on-line technique, the time points for virus infection and harvest were also determined. After virus infection, the OUR continued to increase until it reached a maximum. Subsequently, the OUR decreased rapidly as a result of a decrease of cell metabolic activities or cell lysis caused by virus production. The peak of OUR was correlated to the maximum virus titer. On-line OUR monitoring is easy to implement and is a powerful tool for monitoring the virus infection process.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::284460538c05a6d6ea5d301525d6c78e https://doi.org/10.1016/0141-0229(94)00035-p Zobrazit plný text záznamu Full Text from ScienceDirect