| Autor: |
Vitaly A. Polunovsky, Peter B. Bitterman, Douglas Yee, Anja-Katrin Bielinsky, Shunan Li, J. Carlos Manivel, Alexey O. Benyumov, Kathryn L. Schwertfeger, Thomas C. Beadnell, Jose R. Gomez-Garcia, Mark Peterson, Karen Smith, Jeremy Herrera, Svetlana Avdulov |
| Rok vydání: |
2023 |
| DOI: |
10.1158/0008-5472.c.6507467.v1 |
| Popis: |
Translation initiation factor eIF4E mediates normal cell proliferation, yet induces tumorigenesis when overexpressed. The mechanisms by which eIF4E directs such distinct biologic outputs remain unknown. We found that mouse mammary morphogenesis during pregnancy and lactation is accompanied by increased cap-binding capability of eIF4E and activation of the eIF4E-dependent translational apparatus, but only subtle oscillations in eIF4E abundance. Using a transgenic mouse model engineered so that lactogenic hormones stimulate a sustained increase in eIF4E abundance in stem/progenitor cells of lactogenic mammary epithelium during successive pregnancy/lactation cycles, eIF4E overexpression increased self-renewal, triggered DNA replication stress, and induced formation of premalignant and malignant lesions. Using complementary in vivo and ex vivo approaches, we found that increasing eIF4E levels rescued cells harboring oncogenic c-Myc or H-RasV12 from DNA replication stress and oncogene-induced replication catastrophe. Our findings indicate that distinct threshold levels of eIF4E govern its biologic output in lactating mammary glands and that eIF4E overexpression in the context of stem/progenitor cell population expansion can initiate malignant transformation by enabling cells to evade DNA damage checkpoints activated by oncogenic stimuli. Maintaining eIF4E levels below its proneoplastic threshold is an important anticancer defense in normal cells, with important implications for understanding pregnancy-associated breast cancer. Cancer Res; 75(4); 687–97. ©2014 AACR. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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