| Autor: |
Dylan R. Pillai, Srijak Bhatnagar, Barbara Waddell, Christopher Naugler, Jordan Hollman, Kevin Frankowski, Gopal Achari, Michael D. Parkins, Maria A. Bautista, Nicole Acosta, Janine McCalder, Casey R. J. Hubert, Jenine Leal, Darina Kuzma, Jia Hu, Jon Meddings, Jason Cabaj, Alexander Buchner Beaudet, M. Cathryn Ryan, Jianwei Chen, Norma J Ruecker, Lawrence Man, John Conly, Carmen Li |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.1101/2021.02.20.21251520 |
| Popis: |
BackgroundSARS-CoV-2 has been detected in wastewater and its abundance correlated with community COVID-19 cases, hospitalizations and deaths. We sought to use wastewater-based detection of SARS-CoV-2 to assess the epidemiology of SARS-CoV-2 in hospitals.MethodsBetween August and December 2020, twice-weekly wastewater samples from three tertiary-care hospitals (totaling >2100 dedicated inpatient beds) were collected. Wastewater samples were concentrated and cleaned using the 4S-silica column method and assessed for SARS-CoV-2 gene-targets (N1, N2 and E) and controls using RT-qPCR. Wastewater SARS-CoV-2 as measured by quantification cycle (Cq), genome copies and genomes normalized to the fecal biomarker PMMoV were compared to the total daily number of patients hospitalized with active COVID-19, confirmed cases of hospital-acquired infection, and the occurrence of unit-specific outbreaks.ResultsOf 165 wastewater samples collected, 159 (96%) were assayable. The N1-gene from SARS-CoV-2 was detected in 64.1% of samples, N2 in 49.7% and E in 10%. N1 and N2 in wastewater increased over time both in terms of amount of detectable virus and the proportion of samples that were positive, consistent with increasing hospitalizations (Pearson’s r=0.679, PConclusionsWastewater-based monitoring of SARS-CoV-2 represents a promising tool for SARS-CoV-2 passive surveillance and case identification, containment, and mitigation in acute-care medical facilities.Supplemental Material includedKey-points summarySAS-CoV-2 RNA is detectable in hospital wastewater. Wastewater SARS-CoV-2 RNA increases in conjunction with COVID-19-related hospitalizations. Spikes in SARS-CoV-2 wastewater signal correspond to incident hospital-acquired cases and outbreaks, suggesting passive surveillance via wastewater has great promise for COVID-19 monitoring. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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