Novel Compound from Flowers of Moringa oleifera Active Against Multi- Drug Resistant Gram-negative Bacilli
| Autor: | Samaresh Ghosh, Subhanil Chakraborty, Santi M. Mandal, Smritikona Pyne, Ranadhir Chakraborty, Santanu Sahoo |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Pharmacology Bacilli biology Traditional medicine medicine.drug_class Antibiotics General Medicine biology.organism_classification High-performance liquid chromatography Moringa 03 medical and health sciences Acetic acid chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine chemistry Phytochemical 030220 oncology & carcinogenesis medicine Molecular Medicine Pharmaceutics Antibacterial activity |
| Zdroj: | Infectious Disorders - Drug Targets. 20:69-75 |
| ISSN: | 1871-5265 |
| Popis: | Background: The need for suitable antibacterial agents effective against Multi-drug resistant Gram-negative bacteria is acknowledged globally. The present study was designed to evaluate the possible antibacterial potential of an extracted compound from edible flowers of Moringa oleifera. Methods: Five different solvents were used for preparing dried flower extracts. The most effective extract was subjected to fractionation and further isolation of the active compound with the highest antibacterial effect was obtained using TLC, Column Chromatography and reverse phase- HPLC. Approaches were made for characterization of the isolated compound using FTIR, NMR and Mass spectrometry. Antibacterial activity was evaluated according to the CLSI guidelines. Results: One fraction of aqueous acetic acid extract of M. oleifera flower was found highly effective and more potent than conventional antibiotics of different classes against Multi-drug resistant Gram-negative bacilli (MDR-GNB) when compared. The phytochemical analysis of the isolated compound revealed the presence of hydrogen-bonded amine and hydroxyl groups attributable to unsaturated amides. Conclusion: The present study provided data indicating a potential for use of the flowers extract of M. oleifera in the fight against infections caused by lethal MDR-GNB. Recommendations: Aqueous acetic acid flower extract of M. oleifera is effective, in-vitro, against Gram-negative bacilli. This finding may open a scope in pharmaceutics for the development of new classes of antibiotics. |
| Databáze: | OpenAIRE |
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