Optimization of ultrasound-assisted extraction of phenolic compounds from yarrow (Achillea beibrestinii) by response surface methodology

Autor:	Karamatollah Rezaei, Ahmad Rajaei, Seyyed Ali Mortazavi, Davoud Salar Bashi, Mohamad Mahdi Karimkhani
Rok vydání:	2012
Předmět:	Chromatography Achillea biology Extraction (chemistry) Ultrasound assisted biology.organism_classification Applied Microbiology and Biotechnology Solvent chemistry.chemical_compound chemistry Dry matter Gallic acid Response surface methodology Food Science Biotechnology EC50
Zdroj:	Food Science and Biotechnology. 21:1005-1011
ISSN:	2092-6456 1226-7708
DOI:	10.1007/s10068-012-0131-0
Popis:	An efficient ultrasound-assisted extraction (UAE) technique was applied to extract total phenolic content (TPC) from yarrow (Achillea beibrestinii). A response surface methodology was employed to evaluate the effects of 4 independent variables (temperature, pH, solvent to sample ratio, and time) on the TPC. The optimum extraction conditions were as follows: liquid to solid ratio 20, pH 6.3, extraction temperature 35°C, and extraction time 35 min. Under the above-mentioned conditions, the experimental TPC was 11.7 mg gallic acid equivalent/g dry matter. EC50 of the UAE extract at optimal conditions was found at 30.6±0.6 mg/L level and total flavonoids (TF) were obtained at 12.6±0.2 mg/10 g sample. Also, the results of HPLC analysis showed that gallic acid and p-coumaric acid were the predominant phenolic compounds in the plant.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::280a91ff54886c8608bb3eb6d4696fc3 https://doi.org/10.1007/s10068-012-0131-0 Zobrazit plný text záznamu Full text from SpringerLink