TiO 2 nanoparticle coatings on glass surfaces for the selective trapping of leukemia cells from peripheral blood

Autor: Guido Grundmeier, Rubén Jesús Camargo Amado, Alejandro Gonzalez Orive, Michael Tiemann, Christian Weinberger, Adrian Keller, Sabrina Schwiderek, Jaime Andres Garcia Diosa, Steffen Knust
Rok vydání: 2021
Předmět:
Zdroj: Journal of Biomedical Materials Research Part B: Applied Biomaterials. 109:2142-2153
ISSN: 1552-4981
1552-4973
DOI: 10.1002/jbm.b.34862
Popis: Photodynamic therapy (PDT) using TiO2 nanoparticles has become an important alternative treatment for different types of cancer due to their high photocatalytic activity and high absorption of UV-A light. To potentiate this treatment, we have coated commercial glass plates with TiO2 nanoparticles prepared by the sol-gel method (TiO2 -m), which exhibit a remarkable selectivity for the irreversible trapping of cancer cells. The physicochemical properties of the deposited TiO2 -m nanoparticle coatings have been characterized by a number of complementary surface-analytical techniques and their interaction with leukemia and healthy blood cells were investigated. Scanning electron and atomic force microscopy verify the formation of a compact layer of TiO2 -m nanoparticles. The particles are predominantly in the anatase phase and have hydroxyl-terminated surfaces as revealed by Raman, X-ray photoelectron, and infrared spectroscopy, as well as X-ray diffraction. We find that lymphoblastic leukemia cells adhere to the TiO2 -m coating and undergo amoeboid-like migration, whereas lymphocytic cells show distinctly weaker interactions with the coating. This evidences the potential of this nanomaterial coating to selectively trap cancer cells and renders it a promising candidate for the development of future prototypes of PDT devices for the treatment of leukemia and other types of cancers with non-adherent cells.
Databáze: OpenAIRE
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