The Interaction between Xanthurenic Acid-Insulin Complex and Zinc Ions12

Autor:	Kiyoshi Goda, Edahiko Murakami, Takashi Ueda, Terutoshi Mori, Yahito Kotake
Rok vydání:	1977
Předmět:	Circular dichroism medicine.diagnostic_test chemistry.chemical_element General Medicine Zinc Biochemistry Fluorescence chemistry.chemical_compound chemistry Sephadex Spectrophotometry Excited state medicine Xanthurenic acid Molecular Biology Histidine Nuclear chemistry
Zdroj:	The Journal of Biochemistry. 82:67-72
ISSN:	1756-2651 0021-924X
DOI:	10.1093/oxfordjournals.jbchem.a131693
Popis:	An equimolar mixture of a xanthurenic acid-insulin complex and ZnSO4 was separated into an insulin peak and a peak containing xanthurenic acid (XA) and Zn by Sephadex G-75 column chromatograpy. XA and di-[L-histidino]-zinc (II) readily combined to produce di-[L-histidino]-di-xanthurenato zinc (II) (His2-Zn2+-XA2). By increase in the concentration of Zn2+ ions, XA was removed from di-[L-histidino]-di-xanthurenato zinc (II) (His2-Zn2+-XA2) as XA-Zn2+. The XA-insulin complex showed dcreased relative intensity of fluorescence compared with the Zn-insulin when excited at a wavelength of 284 nm. The difference spectrum between native Zn-insulin and XA-insulin complexes showed a slight red shift. A diffference in the CD spectrum between native Zn-insulin and XA-insulin complexes was observed.
Databáze:	OpenAIRE
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