Isolation and Characterization of an Escherichia albertii Strain Producing Three Different Toxins from a Child with Diarrhea
| Autor: | Takumi Hibino, Akira Nagita, Atsushi Hinenoya, Ayaka Shima, Noritomo Yasuda, Teizo Tsukamoto, Shinji Yamasaki |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) biology Cytolethal distending toxin Sequence analysis 030106 microbiology Shiga toxin General Medicine biology.organism_classification medicine.disease_cause Microbiology Escherichia albertii 03 medical and health sciences 030104 developmental biology Infectious Diseases biology.protein medicine Pathogen Gene Escherichia coli Bacteria |
| Zdroj: | Japanese Journal of Infectious Diseases. 70:252-257 |
| ISSN: | 1884-2836 1344-6304 |
| DOI: | 10.7883/yoken.jjid.2016.186 |
| Popis: | Here, we report a bacterium-isolated as the sole pathogen from a child with diarrhea-harboring eae and 2 different cytolethal distending toxin genes (cdt) that are homologous to Escherichia coli cdt-I and cdt-II. The bacterium was originally identified as atypical E. coli by conventional biochemical testing, but was finally identified as E. albertii by multilocus sequence analysis, which is the only method that can currently differentiate E. albertii from E. coli. The Shiga toxin 2f (stx2f) genes were also detected in the strain. Production of these 3 toxins was confirmed by western blotting and/or a cytotoxicity assay using eukaryotic cell lines. This is the first report showing the biological activity of CDT-I, CDT-II, and Stx2f in E. albertii. |
| Databáze: | OpenAIRE |
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