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Cluster of Differentiation antigen 59 (CD59) is a g lycosyl phosphatidylinositol-linked protein that pr otects cells from damage by lytic terminal complexes of co mplement. Soluble forms of CD59 in serum have been little investigated in disease states. In the prese nt study, by using a sandwich ELISA, reactivities w ith antibodies to CD59 were compared for sera and plasma from normal individuals and those with renal, leukemia, heart, liver and lung diseases. In all of 44 renal patients, reactivities were up to three-f old higher than those of normals and patients with other condi tions. The soluble CD59-related antigen displayed epitopes recognized by an anti-CD59 rabbit antiseru m and CD59 monoclonal antibodies MEM-43 and YTH 53.1. Failure to bind the hydrophobic matrix phenyl Sepharose suggested that the CD59-related antigen lacked a membrane-anchoring structure. Although the cellular origin of soluble CD59-related antigen is unknown, some effect common to different renal diseases can promote increased plasma levels of this antigen. In two individuals, monitored before and a fter renal transplantation, plasma levels of CD59-r elated antigen decreased towards normal after receipt of a kidney. |
