Aerobic methylobacteria as the basis for a biosensor for dichloromethane detection

Autor:	Yu. V. Plekhanova, Nina V. Doronina, Yu. A. Trotsenko, Anatoly N. Reshetilov, Yu. E. Firsova
Rok vydání:	2013
Předmět:	Chromatography biology Chemistry Sorption biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Ancylobacter dichloromethanicus chemistry.chemical_compound Membrane Methylopila helvetica Methylobacterium extorquens Biosensor Nitrocellulose Dichloromethane
Zdroj:	Applied Biochemistry and Microbiology. 49:188-193
ISSN:	1608-3024 0003-6838
DOI:	10.1134/s0003683813020130
Popis:	Cells of dichloromethane (DChM) bacteria-destructors were immobilized by sorption on different types of membranes, which were fixed on the measuring surface of a pH-sensitive field transistor. The presence of DChM in the medium (0.6–8.8 mM) led to a change in the transistor’s output signal, which was determined by the appearance of H+ ions in the medium due to DChM utilization by methylobateria. Among four strains of methylobacteria—Methylobacterium dichloromethanicum DM4, Methylobacterium extorquens DM17, Methylopila helvetica DM6, and Ancylobacter dichloromethanicus DM16—the highest and most stable activity toward DChM degradation was observed in the strain M. dichloromethanicum DM4. Among 11 types of membranes for cell immobilization, Millipore nitrocellulose membranes and chromatographic fiber paper GF/A, which allow one to obtain stable biosensor signals for 2 weeks without a bioreceptor change, were chosen as optimal carriers.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::27c1275c1a25634c09737400643da05b https://doi.org/10.1134/s0003683813020130 Zobrazit plný text záznamu Plný text ve formátu PDF