| Autor: |
Peter J Holt, Hongorzul Davaapil, Deeti K Shetty, Aishwarya G Jacob, Sanjay Sinha |
| Rok vydání: |
2023 |
| DOI: |
10.1101/2023.05.31.543049 |
| Popis: |
The Sinha laboratory has developed protocols for differentiating human pluripotent stem cells (hPSCs) into vascular smooth muscle cells along developmental lineage-specific pathways. In development, paraxial mesoderm (PM), lateral plate mesoderm (LM) and neural crest (NC) linages each give rise to smooth muscle cells significant in a location-specific manner. Induced PSCs derived from patients enduring disease provide a platform from which disease-relevant cell models can be established in the laboratory. Here we describe a robust protocol for differentiating hPSCs into vascular smooth muscle cells via a neural crest lineage and the control steps required to ensure consistently high-quality differentiated cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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