Use of a linear multicopy vector based on the mini-replicon of temperate coliphage N15 for cloning DNA with abnormal secondary structures

Autor:	Ravin Vk, Nikolai V. Ravin
Rok vydání:	1999
Předmět:	Genetics Cloning Plasmid biology Cloning vector Coliphage Replicon P1-derived artificial chromosome pUC19 biology.organism_classification Molecular biology In vitro recombination
Zdroj:	Nucleic Acids Research. 27:i-iii
ISSN:	1362-4962 0305-1048
DOI:	10.1093/nar/27.17.e13-i
Popis:	A new cloning vector pN15L is described. It is a linear 13.8 kb plasmid based on the coliphage N15 mini-replicon. The vector capacity exceeds 50 kb and the copy number is 250 per Escherichia coli chromosome. We show that some artificial and natural palindromes and approximately 5% of human DNA Bgl II fragments can be cloned effectively in linear vector pN15L, whereas they either sharply reduce the copy number of circular vector pUC19 or cannot be cloned at all. We conclude that pN15L may be usefully employed to clone large imperfect palindromes and some abnormal sequences of human DNA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::27b04a8b66e6ecd28fbb54d2a18d8605 https://doi.org/10.1093/nar/27.17.e13-i Zobrazit plný text záznamu