Vesicular stomatitis virus infection reduces the number of active DNA-dependent RNA polymerases in myeloma cells

Autor:	P K Weck, R R Wagner
Rok vydání:	1979
Předmět:	biology RNA RNA-dependent RNA polymerase RNA polymerase II Cell Biology biology.organism_classification Biochemistry Virology Molecular biology Chromatin Vesicular Stomatitis RNA Polymerase Inhibitor Vesicular stomatitis virus biology.protein Molecular Biology Polymerase
Zdroj:	Journal of Biological Chemistry. 254:5430-5434
ISSN:	0021-9258
DOI:	10.1016/s0021-9258(18)50614-1
Popis:	Infection of mouse myeloma (MPC-11) cells with vesicular stomatitis virus resulted in rapid loss in activity of cellular RNA polymerases associated with nuclear chromatin. No RNA polymerase inhibitor could be detected in extracts of infected cell nuclei. Reconstitution experiments with solubilized RNA polymerases dissociated from chromatin of infected and uninfected cells demonstrated that vesicular stomatitis viral infection did not affect the ability of the polymerases to function on endogenous or exogenous templates; nor did infection alter the template capability of the chromatin. Measurement of the number of actively growing RNA chains revealed that infected cell nuclei contained fewer active polymerase units; however, the rates of RNA chain elongation were the same in nuclei from infected and uninfected cells. Quantitation of the number of polymerase units active in nuclear chromatin revealed that the alpha-amantin-sensitive polymerase II was more severely reduced by viral infection than were polymerases I and III.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::27a37f912be41da60edf1b79474752ee https://doi.org/10.1016/s0021-9258(18)50614-1 Zobrazit plný text záznamu