Antibacterial activity of silver nanoparticles (AgNP) confined to mesostructured silica against methicillin-resistant Staphylococcus aureus (MRSA)
| Autor: | Chi-Sheng Chien, Sung-Pin Tseng, Chih-Jung Ko, Chi-Jen Shih, Chao-Jen Lin |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
medicine.drug_class
Chemistry Mechanical Engineering Antibiotics Metals and Alloys 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology medicine.disease_cause 01 natural sciences Methicillin-resistant Staphylococcus aureus Silver nanoparticle 0104 chemical sciences Pulmonary surfactant Mechanics of Materials Staphylococcus aureus Specific surface area Materials Chemistry medicine Agar diffusion test 0210 nano-technology Antibacterial activity Nuclear chemistry |
| Zdroj: | Journal of Alloys and Compounds. 747:1-7 |
| ISSN: | 0925-8388 |
| DOI: | 10.1016/j.jallcom.2018.02.334 |
| Popis: | Prosthetic joint infection (PJI) is one of the main factors that causes joint replacement surgery failure, of which hip joint infection is the most serious. Staphylococcus aureus accounts for more than 50% of the infections caused by pathogenic microorganisms. Unfortunately, antibiotics have long been used to kill bacteria, but increasingly, multiple drug resistant pathogens (MDRP), such as methicillin-resistant Staphylococcus aureus (MRSA), have appeared and are causing a significant reduction in the efficacy of traditional antibiotics. In this study, silver nanoparticles (AgNP) confined to mesostructured silica (MS-Ag) powders were prepared to evaluate their antibacterial activity against MRSA. According to the results of thermogravimetric analysis (TGA), there was no weight loss when the temperature was greater than 600 °C. This result showed that the water, alcohol, non-ionic surfactant F127 and PUF in the silicone-containing colloid were completely removed. These results indicated that the calcination temperature for total dehydration was at least 600 °C. The as-synthesized precursor samples of MS-Ag1 were calcined directly at 400 °C, 500 °C, 550 °C and 600 °C for 2 h. The specific surface area increased over the range of 400–500 °C, and the non-ionic surfactant F127 and PUF were removed over this temperature range. Further, a transition occurred from an amorphous state to a crystalline state, and the thickness of the pore wall increased with the increasing calcination temperature over the range of 500–600 °C. Thus, the specific surface area and pore volume decreased from 526.7 m2/g and 0.84 cm3/g to 384.7 m2/g and 0.67 cm3/g, respectively. The antibacterial activity of MS-Ag powders against MRSA was verified by the disk diffusion method and time-killing curves. The minimum inhibitory concentrations (MIC) of the MS-Ag powders against MRSA were between 2.5 mg/mL and 5 mg/mL. |
| Databáze: | OpenAIRE |
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