Protein and solvent dynamics as studied by QENS and dielectric spectroscopy
| Autor: | Rikard Bergman, Helén Jansson, Jan Swenson |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Physics::Biological Physics
Quantitative Biology::Biomolecules Chemistry Protein dynamics Relaxation (NMR) Dielectric Neutron scattering Condensed Matter Physics Electronic Optical and Magnetic Materials Dielectric spectroscopy Condensed Matter::Soft Condensed Matter Quantitative Biology::Subcellular Processes Mean squared displacement Solvent Crystallography Chemical physics Materials Chemistry Ceramics and Composites Physics::Chemical Physics Solvent effects |
| Zdroj: | Journal of Non-Crystalline Solids. 352:4410-4416 |
| ISSN: | 0022-3093 |
| DOI: | 10.1016/j.jnoncrysol.2006.03.120 |
| Popis: | We have investigated the dynamics of a protein (hemoglobin) in three different solvents by broadband dielectric spectroscopy and quasi-elastic neutron scattering (QENS). The solvents used were water, glycerol and methanol. From the dielectric measurements we were able to extract four relaxation processes, where the fastest process is due to solvent dynamics, and the three slower processes arise from protein motions of a different nature, and hence, visible on different time and temperature scales. The results from the mean square displacement (MSD) obtained by quasi-elastic neutron scattering indicate that the fast and local protein motions are strongly related (slaved) by the fastest and most local solvent motions in the case of water and glycerol. In contrast, for methanol we found the low temperature dynamics are dominated by the CH 3 group rotation, which does not promote any protein dynamics. |
| Databáze: | OpenAIRE |
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