| Autor: |
Chris Denning, Sarah Walsh, Nichola Duckworth, C. Patrick McClure, Tim J. Sloan, Rebecca Tarbox, Andrew V. Benest, Alice Cardall, Angela Downie Ruiz Velasco, Vicki M. Fleming, Gemma Clarke, Claire Seedhouse, Michelle Lister, Jamie L. Thompson, Manjinder Khakh, Jaineeta Richardson, Hannah C. Howson-Wells |
| Rok vydání: |
2020 |
| Předmět: |
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| Popis: |
The COVID-19 pandemic, which began in 2020 is testing economic resilience and surge capacity of healthcare providers worldwide. At time of writing, positive detection of the SARS-CoV-2 virus remains the only method for diagnosing COVID-19 infection. Rapid upscaling of national SARS-CoV-2 genome testing presented challenges: 1) Unpredictable supply chains of reagents and kits for virus inactivation, RNA extraction and PCR-detection of viral genomes 2) Rapid time to result of 2=0.9259). Our results demonstrate that sample treatment can greatly affect the downstream performance of SARS-CoV-2 diagnostic kits, with varying impact depending on the kit. We also showed that one-step heat inactivation methods could reduce time from swab receipt to outcome of test result. Combined, these findings present alternatives to the protocols in use and can serve to alleviate any arising supply chain issues at different points in the workflow, whilst accelerating testing, and reducing cost and environmental impact. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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