APPLICATION OF ZEOLITES FOR IMMOBILIZATION OF GLUCOSE OXIDASE IN AMPEROMETRIC BIOSENSORS
| Autor: | Sergei V. Dzyadevych, Albert Sacco, Burcu Akata Kurç, T.B. Goriushkina |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Detection limit
0303 health sciences biology Chemistry technology industry and agriculture macromolecular substances 02 engineering and technology Amperometric biosensor 021001 nanoscience & nanotechnology humanities Amperometry 03 medical and health sciences biology.protein Glucose oxidase 0210 nano-technology Selectivity Zeolite Biosensor 030304 developmental biology Nuclear chemistry |
| Zdroj: | Sensor Electronics and Microsystem Technologies. 7:36-42 |
| ISSN: | 2415-3508 1815-7459 |
| Popis: | An investigation was performed to evaluate the effect of different zeolites (silicalite, zeolite Y and zeolite Beta with varying Si/Al ratio) on the performance of glucose amperometric biosensor based on immobilized glucose oxidase (GOD). It was observed that detection limit of biosensors based on GOD without zeolite was 0.64 mM of glucose. However, in the case of GOD immobilization with zeolites NH4-Beta-25, Na-Beta and silicalites, biosensors with smaller detection limits within the range of 0.01 to 0.04 mM of glucose were obtained. The study of selectivity of designed biosensors showed that biosensor based on GOD immobilized with zeolite NH4-Beta-25 was much selective comparing with biosensor based on GOD without zeolites. Stability of developed devises also was investigated, and in the case of biosensor with GOD and Silicalite-1 it was higher than for GOD without zeolite. Thus zeolites of different types can be effectively used for GOD immobilization in glucose amperometric biosensors development for optimization of sensitivity, selectivity and stability of these devises. |
| Databáze: | OpenAIRE |
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