Expression of human interleukin-3 (multi-CSF) is restricted to human lymphocytes and T-cell tumor lines

Autor:	David G. Nathan, Bernard Mathey-Prevot, Jennifer Wimperis, Steven C. Clark, Barbara E. Bierer, Colin A. Sieff, Charlotte M. Niemeyer
Rok vydání:	1989
Předmět:	Stromal cell medicine.medical_treatment T cell Immunology Cell Biology Hematology T lymphocyte Biology Biochemistry Peripheral blood mononuclear cell Molecular biology Haematopoiesis Cytokine medicine.anatomical_structure medicine Northern blot Interleukin 3
Zdroj:	Blood. 73:945-951
ISSN:	1528-0020 0006-4971
Popis:	While the cellular sources for granulocyte-macrophage colony- stimulating factor (GM-CSF) are known to be widely distributed among several cell types, interleukin-3 (IL-3) gene expression has been demonstrated in only certain T-cell clones and in blood mononuclear cells stimulated with phytohemagglutinin (PHA) and phorbol-myristate- acetate (PMA). To determine which blood cells were responsible for this expression, we fractionated PHA/PMA-stimulated mononuclear cells and identified T lymphocytes as the source of IL-3 mRNA. Low-level IL-3 expression was detected as well in several stimulated human T-cell lines. Hematopoietic stromal cells such as fibroblasts and endothelial cells could not be induced to express IL-3 mRNA. The kinetics of IL-3 mRNA induction in mononuclear cells and lymphocytes stimulated with PHA/PMA or anti-CD3 monoclonal antibody (MoAb) and interleukin-1 (IL-1) were similar to those observed for GM-CSF expression.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::27638e2e0b5abe575e6edab1099a147d https://doi.org/10.1182/blood.v73.4.945.945 Zobrazit plný text záznamu