A236 ASSOCIATION OF STOOL METABOLOMIC PROFILE AND MICROBIOME COMPOSITION RISK SCORE WITH FUTURE ONSET OF CROHN’S DISEASE

Autor: S Lee, J Raygoza Garay, W Turpin, M I Smith, A Goethel, A Griffiths, P Moayyedi, O Espin-Garcia, G Aumais, C N Bernstein, I Avni-Biron, M Cino, C Deslandres, I Dotan, W El-Matary, B G Feagan, D S Guttmen, H Q Huynh, J Hyams, K Jacobson, D R Mack, J Marshall, A Otley, R Panaccione, M S Silverberg, H Steinhart, D Turner, W Xu, K Croitoru
Rok vydání: 2022
Zdroj: Journal of the Canadian Association of Gastroenterology. 5:127-129
ISSN: 2515-2092
2515-2084
Popis: Background Microbial composition-based risk score (MRS) was recently developed and validated to predict future risk of developing Crohn’s disease (CD) among healthy first-degree relatives (FDR) of CD patients. We hypothesized that stool metabolomic profiles, some of which are linked to the gut microbiome, are associated with future risk of CD. Aims To assess the association of stool metabolomic profile with onset of CD and to determine the correlation between stool metabolites and the MRS Methods Healthy FDR of CD patients were recruited as part of the nested case-control cohort of the CCC-GEM Project. Healthy FDRs who later developed CD (n=56) were matched approximately 1:1 by age, sex, follow-up duration, and geographical location with control FDRs remaining healthy (n=66). Stool metabolomics were assessed using the Metabolon’s DiscoveryHD4™ platform, and the stool microbiome characterised by 16s rDNA amplicon sequencing. We fitted a multivariable conditional logistic regression model on the disease status as a function of individual stool metabolites. We additionally performed Spearman correlation between each stool metabolite and the MRS. Results Among 1,029 stool metabolites that were analyzed, 79 were associated with future risk of CD (p Conclusions Stool metabolite profiles may predict future risk of CD. A subset of these metabolites have significant correlation with the MRS with consistent direction of effect. This may suggest that stool metabolites mediate the putative effect of the gut microbiome on CD risk. Further validation in the full GEM cohort is warranted. Funding Agencies CCC, CIHRThe Leona M. and Harry B. Helmsley Charitable Trust; Kenneth Croitoru is the recipient of the Canada Research Chair in Inflammatory Bowel Diseases; Sun-Ho Lee is a recipient of the Imagine/ CIHR/CAG Fellowship Award; Sun-Ho Lee, Juan Antonio Raygoza Garay, and Williams Turpin are recipients of fellowship awards from the Department of Medicine, Mount Sinai Hospital, Toronto, Canada.
Databáze: OpenAIRE
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