Development of a programmable freezing technique on larval cryopreservation in the Pacific oyster Crassostrea gigas
| Autor: | Penny Miller-Ezzy, Xiaoxu Li, Yibing Liu, Jianguang Qin, Xin Zhan, Jiabo Han, Mark Gluis |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Larva animal structures Cryoprotectant biology Umbo fungi Significant difference 04 agricultural and veterinary sciences Aquatic Science Pacific oyster biology.organism_classification Cryopreservation Hatchery 03 medical and health sciences Animal science 040102 fisheries 0401 agriculture forestry and fisheries Crassostrea 030304 developmental biology |
| Zdroj: | Aquaculture. 523:735199 |
| ISSN: | 0044-8486 |
| DOI: | 10.1016/j.aquaculture.2020.735199 |
| Popis: | This study investigated three vital factors to develop a programmable cryopreservation technique for the larvae of Pacific oyster Crassostrea gigas, namely (1) larval developmental stages; (2) cryoprotectant agents (CPAs) and (3) larval density. The D-larval rate was used as the post-thaw survival indicator, which was calculated as the percentage of larvae that develop into D-larvae after 24 h post-fertilization. The results showed that the post-thaw D-larval rate above 80% was achieved when the 18 h post-fertilization (PF) larvae were cryopreserved with the CPA consisting of 10% ethylene glycol +5% Ficoll +0.2% polyvinylpyrrolidone. No significant difference in the rate of post-thaw D-larvae (P > .05) was found between initial larval density of 4 × 105 mL−1 and 1 × 106 mL−1. The shell length of progenies produced from fresh and cryopreserved larvae had no significant difference at all developmental stages investigated. In addition, similar relative mortality rates were found from umbo larvae (day 8 PF) to spat (day 27 PF) between progenies produced from fresh and cryopreserved larvae. Therefore, the larval cryopreservation technique developed in this study could be used to facilitate breeding programs and hatchery management in Pacific oyster aquaculture industry. |
| Databáze: | OpenAIRE |
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