Direct, On-Site, On-Cartridge Toxicokinetic Sampling Prior to On-Line SPE: A Feasibility Study

Autor: J. A. Ooms, J. Wieling, M. J. Hilhorst, Cornelis Tump
Rok vydání: 2004
Předmět:
Zdroj: Chromatographia. 59
ISSN: 1612-1112
0009-5893
DOI: 10.1365/s10337-003-0170-z
Popis: A recently developed sample introduction method prior to on-line SPE and liquid chromatography, termed “sorbent sampling” [1], was tested for its applicability in bioanalysis. The proof of principle described in this article demonstrates the applicability of sorbent sampling for a pharmacokinetic (PK) study with carbamazepine in rats. In this experiment two rats were dosed with carbamazepine and at several time intervals plasma samples were taken. Every sample was applied to and stored on a sorbent sample cartridge, while the remaining plasma was collected for processing by a conventional autosampler for comparison purposes. Quantitation was performed with and without the use of internal standard and the performance of the methods was verified. Results obtained with the sorbent sampling technique are comparable to those obtained with a conventional method. Sorbent sampling proved to be suitable for PK studies with a few practical challenges left. Although sorbent sampling is not yet commercially available, this feasibility study indicates that the sorbent sampling technique is a promising new method of sampling for bioanalytical purposes in biopharmaceutical R&D, with significant advantages such as zero sample loss, reduced animal or human sampling volumes (and hence reduction of animal usage), reduced risk of carry-over and fully automated sample preparation and analysis.
Databáze: OpenAIRE
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