Biological activated carbon process for biotransformation of azo dye Carmoisine by Klebsiella spp
| Autor: | Tayebe Bagheri Lotfabad, Soheila Yaghmaei, Delaram Poorasadollah, Amir Heydarinasab, Farzaneh Aziz Mohseni |
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| Rok vydání: | 2021 |
| Předmět: |
Chromatography
Anaerobic respiration biology medicine.diagnostic_test Chemistry 0208 environmental biotechnology Biofilm Biosorption 02 engineering and technology General Medicine 010501 environmental sciences biology.organism_classification 01 natural sciences High-performance liquid chromatography 020801 environmental engineering Biotransformation Spectrophotometry medicine Environmental Chemistry Waste Management and Disposal Bacteria 0105 earth and related environmental sciences Water Science and Technology Activated carbon medicine.drug |
| Zdroj: | Environmental Technology. 43:2713-2729 |
| ISSN: | 1479-487X 0959-3330 |
| Popis: | The feasibility of employing the biological activated carbon (BAC) process to debilitate azo dye Carmoisine by Klebsiella spp. was investigated. Plate assay revealed the capability of Klebsiella spp. for removal of Carmoisine via degradation. Kinetic parameters were measured for Carmoisine debilitation by Klebsiella spp. using the suspended anaerobic process. Two types of granular and rod-shaped activated carbon were used to form the biological beds in order to study the Carmoisine debilitation in batch processes. Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) were used to indicate the colonization and biofilm formation of bacteria grown on activated carbon particles (ACPs). Thin-layer chromatography (TLC), liquid chromatography-mass spectrometry (LC-MS), high-pressure liquid chromatography (HPLC) and biosorption studies demonstrated biotransformation of Carmoisine into its constituent aromatic amines during the Carmoisine debilitation in suspended anaerobic and BAC processes. The porosity of activated carbons, inoculation size and age of biological beds were the important factors affecting the viability of bacterial cells grown on ACPs and, consequently, the rate and efficiency of the Carmoisine debilitation process determined through spectrophotometry. The reusability of biological beds was demonstrated by conducting sequential batch experiments. In conclusion, the BAC process proved to be an efficient method for anaerobic dye degradation. |
| Databáze: | OpenAIRE |
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