Effect of storage of placenta tissue at –20°C on properties of its aqueous and saline extracts

Autor:	Yana Cherkashina, O. A. Nardid, E. D. Rozanova, Eduard O. Nardid, Svetlana V. Repina
Rok vydání:	2014
Předmět:	Aqueous solution Chromatography Chemistry Biophysics Medicine (miscellaneous) Fraction (chemistry) law.invention Spin probe Microviscosity Cytosol medicine.anatomical_structure Biochemistry law Placenta medicine Spectroscopy Electron paramagnetic resonance
Zdroj:	Problems of Cryobiology and Cryomedicine. 24:28-37
ISSN:	2518-7376 2307-6143
DOI:	10.15407/cryo24.01.028
Popis:	Research was aimed to study the effect of human placenta tissue storage at –20°C on the features of procured from it aqueous-saline extracts and their certain fractions. The methods of gel-chromatography, optical spectroscopy and spin probe EPR were used in the research. Spin probe EPR demonstrated the exposure of erythrocytes with fraction of human placenta extracts below 5 kDa as not affecting the microviscosity of erythrocyte cytosol. Along with that we observed the modification of temperature dependence of spin probe mobility under exposure with the same fraction of placenta extracts, stored for 6 months at –20°C. This correlated with a decrease in acid resistance of erythrocytes under the effect of this fraction. Probl Cryobiol Cryomed 2014; 24(1):28–37.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::272ea3939a1e9e9ba8d830b6f5760ebc https://doi.org/10.15407/cryo24.01.028 Zobrazit plný text záznamu