Determination of the extracellular acidification ofEscherichia coliK12 with a multi-chamber-based LAPS system
| Autor: | Torsten Wagner, Shahriar Dantism, Michael J. Schöning, Shoko Takenaga, Patrick Wagner |
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| Rok vydání: | 2016 |
| Předmět: |
Microorganism
Analytical chemistry 02 engineering and technology medicine.disease_cause 01 natural sciences Reference electrode Materials Chemistry medicine Potentiometric sensor Electrical and Electronic Engineering Escherichia coli Biogas production Chromatography Chemistry 010401 analytical chemistry Surfaces and Interfaces 021001 nanoscience & nanotechnology Condensed Matter Physics Differential measurement 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials bacteria 0210 nano-technology Metabolic activity Extracellular acidification |
| Zdroj: | physica status solidi (a). 213:1479-1485 |
| ISSN: | 1862-6300 |
| DOI: | 10.1002/pssa.201533043 |
| Popis: | On-line monitoring of the metabolic activity of microorganisms involved in intermediate stages of biogas production plays an important role to avoid undesirable “down times” during the biogas production. In order to control this process, an on-chip differential measuring system based on the light-addressable potentiometric sensor (LAPS) principle combined with a 3D-printed multi-chamber structure has been realized. As a test microorganism, Escherichia coli K12 (E. coli K12) were used for cell-based measurements. Multi-chamber structures were developed to determine the metabolic activity of E. coli K12 in suspension for a different number of cells, responding to the addition of a constant or variable amount of glucose concentrations, enabling differential and simultaneous measurements. Multi-chamber differential measurement setup based on the LAPS principle; RE, reference electrode. |
| Databáze: | OpenAIRE |
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