Potentials of a Synchronized Culture of Tetrahymena pyriformis for Toxicity Studies of Mycotoxins

Autor: Jan P Bijl, Daniel Dive, Carlos Van Peteghem, D. M. Rousseau
Rok vydání: 1988
Předmět:
Zdroj: Journal of AOAC INTERNATIONAL. 71:282-285
ISSN: 0004-5756
DOI: 10.1093/jaoac/71.2.282
Popis: A synchronized culture of Tetrahymena pyriformis is proposed for toxicity testing of mycotoxins. Toxicity is evidenced through a delay in the division of the synchronized culture. Newly developed equipment for the synchronization consists of a double-walled culture flask built in an alternating water circuit at 28°C (optimum) and 34°C (sublethal), respectively, controlled by a time switch. The cultures are used for the detection of 16 mycotoxins for which the detection threshold is given. Synchronous cultures of Tetrahymena pyriformis appear to be very suitable for the detection of tr ichothecenes. Preliminary experiments on artificially contaminated corn samples demonstrate the possibility of prescreening of cereals
Databáze: OpenAIRE