LC Determination of Four Isoflavone Aglycones in Red Clover (Trifolium pratense L.)

Autor: Graziele Pereira Ramos, Pedro Eduardo Fröehlich, Cláudia Borges de Morais, José Angelo S. Zuanazzi, Paula Menna Barreto Dias, Miguel Dall'Agnol
Rok vydání: 2007
Předmět:
Zdroj: Chromatographia. 67:125-129
ISSN: 1612-1112
0009-5893
Popis: Red clover (Trifolium pratense L.) is an important forage plant that contains the isoflavones daidzein, genistein, formononetin, and biochanin A. These compounds have been studied lately due to their human health benefits. The aim of this study was to develop and validate an HPLC method with simplified sample preparation to quantify daidzein, genistein, formononetin and biochanin A simultaneously in red clover leaves. The validation showed that the method is specific, accurate, precise and robust, not to mention that the sample preparation is easier and faster than those described earlier. The response was linear over a range of 0.01–0.2 μg mL−1 for daidzein, 0.05–0.5 μg mL−1 for genistein, 4–40 μg mL−1 for formononetin and 2–20 μg mL−1 for biochanin A. The range of recoveries was 85.6–101.0%. The RSD for intra- and inter-day precision were
Databáze: OpenAIRE
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